Triton wr 39 has been widely used to block clearance of tgrich lipoproteins to induce acute hyperlipidemia in several animals 18. Minimum rates of triglyceride entry into the plasma have been calculated from measurements of the plasma triglyceride concentration at 15 hr after triton. Trout, jerome joseph, morphological, cytochemical and biochemical effects of triton wr 39 on rat hepatocytes 1977. Triton wr 39, thiobarbituric acid tba, 2,4, dinitrophenylhydrazine dnph, reduced glutathione and triton wr 39 were purchased for sigma chemical company, mumbai. Hypolipidemic effect of trixis angustifolia aqueous extract on triton. Neither compound influenced subcutaneous tumour development or reduced the propensity to metastasize when administered in regimens reported to suppress pulmonary, lymph node or. Tetrahydrolipstatin decreased the accumulation of newly synthesized, and of total triacylglycerol in the medium. View enhanced pdf access article on wiley online library html view download pdf for offline viewing. Triton wr 39, a nonionic detergent that inhibits lipoprotein catabolism, was used to estimate hepatic lipid secretion by measuring the increment in serum lipids after its administration. When leucocytes from tuberculinsensitive guinea pigs are exposed to triton in vitro at a. Lp fraction and subfraction compositions were compared by smallangle xray scattering on a diffractometer. The synthetic ethinyl estradiol has been shown to depress bile flow in rats simon eta.
Results in tables 1, 2 and 3 indicated that the treatment with triton wr 39 caused a significant p triton wr 39 twr, a formaldehyde polymer of polyoxyethylene ether of octylphenol, decreases the formation of lung metastases in c 57 b110 jsel mice transplanted in the leg with lewis lung carcinoma, it reduces the number of lymph node metastases in swiss albino mice transplanted intratibially with ehrlich carcinoma and it reduces the number of cancer cells in the lung of swiss albino. Protection of saururus chinensis extract against liver. In vitro and in vivo interactions of triton 39 with. Administration of triton wr 39 to guinea pigs 300 mgkg altered plasma lipoproteins so that they were not cleared by guinea pig lipoprotein lipase. Basbscr54 the hypolipidaemic activity of novel indole2. The tracer and nontracer methods showed that fructosefed rats had a significantly lower fractional catabolic rate fcr than either control or glucosefed rats. The pharmacological effects of novel 5fluoro n 9,10dihydro9,10dioxoanthracen8yl1 h indole2carboxamide derivatives on plasma lipid profile of triton wr 39 induced wistar rats 31 may 2012 journal of enzyme inhibition and medicinal chemistry, vol. Jun 27, 20 lipemia modeled by injections of triton wr 39 500 mgkg and poloxamer 407 500 mgkg to mice were compared. Effect of ethinyl estradiol and triton wr 39 on bile. Antihyperlipidemic activity of michelia champaca l. Pdf lipid lowering activity of a polyherbal formulation. Triton wr 39 had no significant effect on the eeinduced changes or on bsptm.
Hypolipemic activity of polyphenolrich extracts from ocimum basilicum in triton wr 39 induced hyperlipidemic mice. Phenolic extract from ocimum basilicum restores lipid metabolism in triton wr 39 induced hyperlipidemic mice and prevents lipoproteinrich plasma oxidation. Pharmacological evaluation of convolvulus pluricaulis as. Lipoprotein lipase activity was measured in heart homogenates and in heparinreleasable and nonreleasable fractions of isolated perfused rat hearts, after the intravenous injection of triton wr 39. Antihyperlipidaemic activity of spermacoce hispida. At 15h after triton wr 39 administration, serum cholesterol concentrations were increased equally in both control and ethynyloestradioltreated rats. Materials and methods measurement of bacillus subtilis growth. Pdf antihyperlipidemic activity of cassia auriculata. In the triton model, treatment with txae at 100 and 200 mgkg body weight produced a significant decrease in triglycerides and very lowdensity lipoprotein levels and a significant increase in highdensity. In homogenates of hearts from starved, rats, lipoprotein lipase activity was significantly inhibited p less than 0.
Using triton wr 39 induced hyperlipidaemic rats as an experimental model, we investigated whether compound 1 n3benzoylphenyl1hindole2carboxamide and 2 n4benzoylphenyl1hindole2carboxamide novel antihyperlipidaemic agents have any effect on plasma triglyceride, total cholesterol tc and highdensity lipoprotein cholesterol levels hdlc levels. Triton wr 39 twr, an inhibitor of cancer dissemination. Comparison of the isotopical tracer and the triton wr 39. Triton, which raised serum very low density lipoprotein. Catalase activity decreased to about 35% of that of the control at 4248 h after the injection and recovered to the normal level at 96 h.
Pdf hypolipidemic and antioxidative effects of dika nut. Hypolipidemic effect of trixis angustifolia aqueous extract. Induction of hyperlipidaemia hyperlipidaemia was induced in wistar rats by intraperitoneal i. The aim of the study was to evaluate the effect of convolvulus pluricaulis cp. When administered to ethinyl estradioltreated rats, triton wr 39 restored bile salt independent bile flow and maximum taurocholate transport, whereas phenobarbital. Objectiveto determine whether a nonionic detergent triton wr 39 can be used in cats to assess hepatic secretion of triglyceride. Triton wr 39 and orotic acid were administered to rats, and the biliary cholesterol, phospholipids and bile acids were analyzed together with serum lipoproteins and hepatic lipids.
Post triton vldl production rate in fasting rabbits at 8 weeks after hffd or chow diet feeding was measured. Inhibition of proteolytic degradation of low density. Pdf triton wr 39 an inhibitor of lipoprotein lipase. Triton wr 39 twr, a formaldehyde polymer of polyoxyethylene ether of octylphenol, decreases the formation of lung metastases in c 57 b110 jsel mice transplanted in the leg with lewis lung carcinoma, it reduces the number of lymph node metastases in swiss albino mice transplanted intratibially with ehrlich carcinoma and it reduces the number of cancer cells in the lung of swiss albino. Another group of rats was anesthetized with diethyl ether and. Pretreatment with triton wr 39 also partially increased cc14induced peroxidation of microsomal lipids at 1, 3 or 6 h after administration of the hepatotoxin. Hypolipidaemic activity of orally administered diphenyl diselenide in triton wr 39 induced hyperlipidaemia in mice.
The objective of this study was to investigate the hypolipidemic properties of chrysin on triton wr 39 induced hyperlipidemia in female c57bl6 mice. Two other agents affecting lysosomal function, triton wr 39 and concanavalin a, also inhibited the proteolytic degradation of low density lipoprotein in intact fibroblasts and simultaneously prevented low density lipoproteinmediated suppression of 3hydroxy3methylglutaryl coenzyme a reductase activity and stimulation of cholesteryl ester. Morphological, cytochemical and biochemical effects of triton. Improved isolation procedures, automated analysis, biochemical and morphological properties of fractions. Triton wr39induced hyperlipidemia, dna fragmentation. In view of these results and the fact that the density of triton wr 39 is quite high at least d 1.
Phenolic extract from ocimum basilicum restores lipid. Use of a nonionic detergent triton wr 39 in healthy cats. The increase in cholesterol is confined to the blood itself. Effects of triton wr39 on lipoprotein lipolytic activity. Hypolipemic activity of polyphenolrich extracts from. The ratios of hdlctc and hdlcldlc were increased to 0. Proceduretriton wr 39 was administered iv according to the following schedule. Triton wr 39 was incubated in vitro in various proportions with plasma from normolipidemic rhesus monkeys or with ultracentrifugally purified lipoproteins, and the products were examined by isopycnic density gradient ultracentrifugation, agarose column chromatography, electrophoretic and immunochemical techniques, and electron microscopy. The effect of triton wr39 on the rates of synthesis and degradation of hepatic catalase was examined. In order to investigate the effect of hepatic cholesterol flux on biliary bile acids, triton wr 39 and orotic acid were administered to rats, and the biliary cholesterol, phospholipids and bile acids were analyzed together with serum lipoproteins and hepatic lipids. Pdf antihyperlipidemic activity of alcoholic extracts of. This occurs in rat liver lysosomes2 and to some extent in kidney3 and spleen4 lysosomes after injection of triton wr39, a nonionic detergent.
At 24 h after triton wr 39 injection, blood samples were collected to. The results were controlled biochemically by differential and linear density gradient ultracentrifugation of extracts from normal and. Pdf use of a nonionic detergent triton wr 39 in healthy cats. Introduction administration of estrogens may interfere with bile formation in animals and men plaa and pristly, 1977. Hypercholesterolemia is a dominant risk factor for atherosclerosis and cardiovascular diseases. The large increase in plasma tc and tg due to triton administration results mostly. Pdf hypolipidaemic activity of orally administered.
At 24 h after triton wr 39 injection, blood samples were collected to measure plasma lipid levels. Federico leighton, brian poole, henri beaufay, pierre baudhuin, john w. Phenolic extract from ocimum basilicum restores lipid metabolism in triton wr39. Triton wr 39 was administered intraperitoneally 400 mgkg to overnightfasted mice to develop acute hyperlipidemia. Hypolipidemic effect of flavonoids from mulberry leaves in. The aim of the present study was to evaluate the hypolipidemic effect of trixis angustifolia aqueous extract txae on triton wr 39 and highfat dietinduced hyperlipidemic mice. Triton wr 39 modifies tg catabolism by inhibition of lipoprotein lipase lpl lipolytic activity. Treatment with arogyavardhini vati 50, 100, 200 mgkg in triton wr 39 induced dyslipidemic rats significantly decreased ai level in a dosedependent manner when compared with triton treated rats p triton wr 39 induced wistar rats 31 may 2012 journal of enzyme inhibition and medicinal chemistry, vol. The concentration of triglyceride in the plasma rises linearly for 3 hr after the intravenous injection of the nonionic detergent, triton wr 39, in the rat. Methods the study comprised of six groups namely normal control, experimental control and treatment groups 100, 200 and 400 mgkg of c. The present study was undertaken to assess the lipid lowering activity of methanolic extract from feronia limonia leaf in triton wr 39 induced hyperlipidemic rats.
Sixty male albino rats were randomly and equally divided into four groups. Pdf triton wr39induced hyperlipidemia, dna fragmentation. To determine whether a nonionic detergent triton wr 39 can be used in cats to assess hepatic secretion of triglyceride. The effects of triton wr 39 and phenobarbital on ethinyl estradiol bile secretory failure were examined to determine the mechanism responsible for decreased bile salt excretion. Pdf to determine whether a nonionic detergent triton wr 39 can be used in cats to assess hepatic secretion of. Hyperlipidemia was induced via intraperitoneal injection of triton wr. Hypolipidemic action of chrysin on triton wr39induced. Pdf use of a nonionic detergent triton wr 39 in healthy. Effects of triton wr 39 on lipoprotein lipolytic activity and lipid content of rat liver lysosomes. Plasma lipoproteins of normal guinea pigs similarly were rendered resistant to lipoprotein lipase by incubation with triton in vitro. Pdf lipid lowering activity of feronia limonia leaf in. Hyperlipidemia was induced in rats by a single intraperitoneal.
Rats were administered an oral gavage of 1 ml of vitamin efree emulsion control, n 4, emulsion containing 10 mg. Influence of icrf 159 and trition wr 39 on metastases of. Minimum rates of triglyceride entry into the plasma have been calculated from measurements of the plasma triglyceride concentration at 15 hr after triton injection. All other chemicals and reagents used in this study were of analytical grade with high purity and were obtained from glaxo laboratories and. In incubations of triton with separated lipoproteins, the protein concentration.
Triton wr 39 increased the recovery of triacylglycerol in the hepatocytes and incubation medium by 31% and 38%, respectively. Effect of phoenix dactylifera seeds dates extract in triton wr39 and high fat diet induced. Influence of the injection of triton wr39on lysosomes of a rat. Icrf 159 and triton wr 39 have been examined for their ability to suppress subcutaneous growth and pulmonary metastases from a transplanted rat epithelioma. Hyperlipidemia is characterized by elevated serum total cholesterol, lowdensity cholesterol, and very low density lipoprotein cholesterol and. Use of triton wr39 in cytochemical and biochemical. A new cytochemical assay of acid phosphatase activity employing triton wr 39, a nonionic detergent has been used to demonstrate the probable origin of the phytolysosome system and of the organization of the golgi complex in the root meristem,cucurbita pepo. The characteristics of lipoprotein lipolytic activity in lysosomes after the administration of triton wr 39 were studied, and the observed decrease in the density of the particles is discussed. Effect of triton wr39 on the rates of synthesis and. Hypolipidemic effect of trixis angustifolia aqueous.
Chrysin was administered orally 10 mgkg 30 min before triton wr 39. Triglyceride contents of total plasma c, top and vldls d triton wr 39 and phenobarbital on ethinyl estradiol bile secretory failure were examined to determine the mechanism responsible for decreased bile salt excretion. Mechanism underlying hypercholesteremia induced by triton wr39. Triton wr 39 and tetrahydrolipstatin, were incubated with rat hepatocytes. The hypolipidemic activity of michelia champaca extract was studied on triton wr 39 induced models of hyperlipidemia in rats. Pdf on jul 9, 2014, anil pareek and others published effect of atorvastatin and hydroxychloroquine combination on triton wr 39 induced hyperlipidemia in rats find, read and cite all the. Triton, which raised serum very low density lipoprotein and lipid levels and decreased serum high density lipoprotein liver. Reversal of ethinyl estradiolinduced bile secretory failure. Injection of triton wr 39 into rats leads to a rapid increase in the cholesterol, cholate, and various lipid fractions of their blood. This occurs in rat liver lysosomes2 and to some extent in kidney3 and spleen4 lysosomes after injection of triton wr 39, a nonionic detergent. Highfructose and highfat dietinduced insulin resistance. Both compounds in the same dose caused a sharp increase in serum concentrations of total cholesterol ch and triglycerides tg, the increases in response to poloxamer 407. Antihyperlipidemic activity of cassia auriculata flowers in triton wr 39 induced hyperlipidemic rats.
Control cats did not receive an injection or received 0. Reversal by triton wr39 of ethynyloestradiolinduced. Antihyperlipidemic activity of alcoholic extracts of trichosanthes anguina in triton wr 39 induced hyperlipidemic rats. Effects of triton wr 39 and soybean oil and their combination on lipid peroxidation and antioxidant enzymes. Lipid lowering activity of a polyherbal formulation on triton wr 39 tyloxapol induced hyperlipidemia in wistar rats. Antihypercholesterolemic and antioxidative potential of an. Effect of phoenix dactylifera seeds dates extract in triton wr39. Influence of the injection of triton wr39 on lysosomes of a rat transplantable hepatoma. Effect of arogyavardhini vati in triton wr 39 induced dyslipidemia on atherogenic index level in rats. A single injection of triton wr39 in mice group ii caused a marked increase of serum triglyceride tg and very lowdensity lipoprotein. The results were controlled biochemically by differential and linear density gradient ultracentrifugation of extracts from normal. This is a temporary file and hence do not link it from a website. Effect of triton wr39 on lipoproteins and lipoprotein. Hypolipidemic action of chrysin on triton wr39induced hyperlipidemia in female c57bl6 mice micheli stefani zarzecki, stifani m.
Triton wr 39, a potent inhibitor of vldltg catabolism, was injected into the same animal 30 min after the end of the tracer method to measure tg secretion rate tgsr. In the triton model, treatment with txae at 100 and 200 mgkg body weight produced a significant decrease in triglycerides and very lowdensity lipoprotein levels. Comparative characteristics of lipemia models induced by. The inhibition in vivo of lipoprotein lipase clearingfactor. The cholesteremic effect of triton was not dependent upon a prior accumulation of cholate in plasma. Pdf antihyperlipidemic activity of michelia champaca l. American journal of medical and biological research, 3, 146148. Although the sensitivity of guinea pig leucocytes to thermal shock remains unchanged after a single injection of triton, their resistance to disruption by acetone and by sonic vibration is increased. Pdf abstract background triton wr39 tyloxapol is the nonionic detergent, which is able to increase the. Triton wr 39 a nonionic detergent, isooctyl poly oxyethylene phenol was obtained from sigma chemicals co, mumbai. Liver concentrations of cc14 in triton wr 39 treated rats were significantly higher at 3. Triton wr39 was injected intraperitoneally into rats at a dose of 200 mg per 100 g body weight. Hyperlipidemia in experimental rats evidenced by an enhancement in the levels of cholesterol, triglycerides.